May 28, 2020  
Spring 2019 Catalog 
Spring 2019 Catalog [ARCHIVED CATALOG]

ML 229 - Clinical Microbiology II Lab

Credit Hours: 1.0

Continuation of ML219 with advanced techniques in procedures for cultivation, isolation and identification of pathogenic microorganisms from a variety of hospital provided or simulated clinical specimens. Clinical specimens include urine samples, throat, rectal, genital and wound swabs.

Course Outcomes
Upon the completion of this course the student will be able to:

  • perform and read gram-stained smears on hospital specimens;
  • inoculate and incubate appropriate culture media for the following clinical specimens: urine, throat, genital, rectal, and wound swabs;
  • identify the pathogens in hospital specimens and perform designated confirmatory test procedures;
  • set up and read antibiotic susceptibility testing plates using the Kirby Bauer technique;
  • observe hospital-provided demonstration gram stains and plates of sputum blood, and cerebrospinal fluid cultures;
  • culture anaerobic organisms and identify microscopic morphology of common isolates;
  • identify common pathogenic fungi including both yeast and molds macroscopically and microscopically;
  • use slide agglutination and ELISA test kits for rapid identification of Staphylococcus aureus, Strepococcus pyogenes, and Streptococcus agalactiae;
  • follow the laboratory safety rules stated in the performance of test procedures in the clinical microbiology laboratory; and
  • use internet to access the site for additional reference and review materials in clinical microbiology

Prerequisites: ML218, ML219
Corequisites: ML228
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