BT 222 - Laboratory Calculations

Credit Hours: 1

This course is intended for members of the health, biotechnology and quality and laboratory professions, with potential responsibility for solution preparation, laboratory testing, instrument calibration and quality control calculations. This course will cover the calculations necessary for determination of laboratory concentrations, solutions and variation, calibration, quality control and reference intervals. The basic units of analytical instrumentation, pH calculations, buffer solutions, spectroscopy, basic statistics and graphing for quality control reference ranges will be discussed.

Course Outcomes
Upon completion of this course, the student will be able to:

calculate both concentrations and content of solutions in the concentration units used in the biotechnology, biomanufacturing and health professions;
perform dilution calculations when given appropriate information;
calculate the volumes of various stock solutions required to make working solutions for analysis and to make solutions of acids and bases;
calculate the standard deviation, mean, median, mode and coefficient of variation;
apply the terms accuracy, precision, reliability, confidence intervals and relate these terms to laboratory measurement and determination of reference ranges;
apply setting up, graphing and interpretation of quality control charts and calculation of reference ranges;
perform simple pH and chemical buffer system calculations using the Henderson-Hasselbalch buffer equation;
apply Beer’s Law to perform calculations of absorbance and transmittance for solution concentration in spectroscopy; and
determine concentration by calculation of standard concentration and calibration curves for spectroscopy and colorimetric analysis

Prerequisites: Math placement at MT125 or higher
S/F (N)