ML 217 - Lab for ML 216

Credit Hours: 1.5

The student will practice procedures routinely performed in a transfusion service including ABO and Rh, antiglobulin and compatibility testing. The student must achieve a minimum grade of 80 percent on the final practical exam in order to pass the course. (Note: a critical mistake (ABO-Rho) during the final practical/competency will result in automatic failure in the course).

Course Outcomes
Upon completion of this course, the student will be able to:

show proficiency in the operation of mechanical equipment;
explain and understand routine quality control procedures;
prepare a 2-5% suspension of red blood cells with proficiency;
show the ability to grade agglutination reactions with consistency and proficiency;
recognize reactions involving hemolysis and mixed field agglutination;
explain the type of samples acceptable for testing in the laboratory and when and why they are used;
perform routine ABO-Rho testing, tube method, and interpret results accurately;
perform the testing for “weak D” (Du) and interpret the results accurately;
utilize Coombs Control Cells (Check Cells or CCC) and explain their importance in antiglobulin testing;
explain the testing procedure necessary for distinguishing between A1 and A2;
discuss RH-Hr testing and interpret the results accurately;
interpret the RH-Hr phenotype/genotype accurately using both Fisher-Race and Wiener nomenclature;
perform antiglobulin testing, both the direct and indirect methods, using polyspecific (“Broad Spectrum”) reagents and interpret the results accurately;
perform the antibody screening procedure and interpret the results accurately;
perform the donor compatibility testing procedure (Crossmatch) and interpret results accurately; and
resolve simple incompatibilities; understanding the cause and solutions.

Prerequisites: ML 124
Corequisites: ML 216
F (N)