ML 219 - Clinical Microbiology I Lab

• Safety and Microscope:
  • discuss and follow the safety regulations in the microbiology laboratory;
  • demonstrate proper care in handling the microscope;
  • locate and describe the function of the parts of the compound light microscope;
  • use the microscope to observe prepared slides of blood cells; and
  • complete laboratory reports as assigned.
• Bacterial Cell Morphology:
  • use the microscope to examine prepared slides of various microorganisms;
  • use correct bacteriological terminology to describe shapes and arrangements of bacteria; and
  • complete laboratory reports as assigned.
• Media Preparation:
  • calculate the number of grams of dehydrated medium needed to prepare a specified volume of medium;
  • demonstrate the proper use of the balance for weighing out dehydrated powdered medium;
  • use a graduated cylinder to measure out specified volumes of water to dissolve the medium;
  • dissolve, dispense, and sterilize media as specified; and
  • complete laboratory reports as assigned.
• Aseptic Technique:
  • describe procedures and principles of aseptic technique practiced in the microbiology laboratory;
  • use aseptic technique to transfer broth, agar slant, and agar plate cultures;
  • demonstrate proper technique for a streak plate culture for isolation of bacteria;
  • culture bacteria from various environmental sites; and
  • complete laboratory reports as assigned.
• Examination of Cultures:
  • describe characteristics of microbial cultures;
  • examine cultures prepared by students and use proper terminology to describe colony characteristics; and
  • complete laboratory reports as assigned.
• Smear Preparation and Staining:
  • prepare a wet mount, observe motility, and distinguish motility from Brownian movement and streaming;
  • describe the procedure for preparing smears from broth and agar media;
  • properly prepare smears from various specimens;
  • perform a simple stain and examine the smear;
  • perform a Gram stain using control cultures to check for accuracy and examine the smear;
  • describe other special stains and preparations and examine prepared smears; and
  • complete laboratory reports as assigned.
• Control of Microbial Growth:
  • test the effectiveness of various antiseptic and disinfectant chemicals against test bacteria and interpret the results;
  • observe demonstration plates of antimicrobial susceptibility tests and interpret the results; and
  • complete laboratory reports as assigned.
• Clinical Microbiology - Upper Respiratory Tract:
  • obtain a nasal and throat specimen for culture;
  • examine cultures and describe colony morphology;
  • differentiate Staphylococcus and Streptococcus genera by microscopy and catalase testing;
  • speciate Staphylococcus and Streptococcus by morphology and biochemical tests; and
  • complete laboratory reports as assigned.
• Clinical Microbiology - Genitourinary Tract:
  • describe urinary samples for culture;
  • obtain a urine specimen for culture;
  • examine and interpret urine colony counts;
  • describe biochemical tests for differentiation of enteric bacteria;
  • observe demonstration slides of gonorrhea prepared from clinical specimens; and
  • complete laboratory reports as assigned.
• DNA Fingerprinting:
  • develop an understanding of the basic scientific principles in the DNA fingerprinting methodology; and
  • weigh evidence, analyze, and interpret data generated in this procedure.
• Mycology:
  • isolate fungi from the environment;
  • describe the classification of fungi and give examples of representative yeasts and molds;
  • observe macroscopic and microscopic morphology of representative yeasts and molds; and
  • complete laboratory reports as assigned.
• Bacterial Unknown Identification:
  • inoculate appropriate plating media for assigned unknown organism or specimen;
  • incubate unknown using proper temperature and atmospheric conditions;
  • observe bacterial growth and perform Gram stain on isolated colonies;
  • determine and inoculate appropriate biochemical test media for organism identification; and
  • complete and hand in lab report.

 

Prerequisites: BI 147, BI 148
Corequisites: ML 218
F (N)