BT 210 - Immunoassay Analysis

Credit Hours: 3

This course will introduce the student to the concepts and procedures used in immunoassay testing. These concepts will be practiced in a separate weekly laboratory section. Students will be introduced to the basics of proteins, nucleic acids (DNA and RNA) including levels of structure, function and assay principles and concepts to characterize and quantitate. Students will explore electrophoresis, several different methods of immunoassay, chromatography, and spectrophotometry. Quality control concepts will be covered including acceptance criteria and troubleshooting techniques.

Course Outcomes
Upon completion of this course, the student will be able to:

explain basic immunoassay concepts and techniques;
describe the structure and preparation of antibodies;
define categories of immunoassay methodologies;
exhibit comprehension of protein, and nucleic acids, levels of structure, functions, and methods of analysis to characterize and determine concentration;
demonstrate proficiency of concepts and principles of protein separation in electrophoresis procedures, quality control methods and troubleshooting separation pattern issues;
demonstrate comprehension of spectrophotometric analysis procedures and quality control;
perform calculations for concentration, absorbance, and percent transmittance;
demonstrate proficiency in quality control principles of calibration, criteria for acceptance of a run and creation of a calibration or concentration curve as well as Westgard rules of quality control system; and
demonstrate comprehension of chromatographic procedures, principles and analytic methods.

Prerequisites: CH 180, CH 181
Corequisites: BT 211
F (N)