BT 240 - Production of BioPharmaceutical Products

Credit Hours: 3

Understanding protein function is essential to biological research and operations. Protein complexity and large numbers of proteins in the cell make it difficult to identify proteins of interest or protein purification. This course will discuss protein structure and functions as well as introduce the student to protein purification methods or protein-protein interaction. These methods include gel electrophoresis, column chromatography and methods preparation of cellular and subcellular extracts, essential to protein purification.

Also discussed in the course are methods for characterizing protein-protein interactions, essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. The course culminates with cell culture basics, purpose, cell lines, and culture maintenance.

Course Outcomes
Upon completion of this course, the student will be able to:

accurately characterize the structure and functions of proteins;
specify the aseptic processing requirements for cell culture in biotechnology;
demonstrate understanding in the performance and methodology of selected standard protein assays utilized in biotechnology;
characterize the strategies used in protein purification; and
analyze case studies of typical products developed in the biotechnology industry.

Prerequisites: BT 210 /BT 211
Corequisites: BT 241
S online (N)