BI 213 - Laboratory for BI 212

Credit Hours: 2

Two 2-hour periods of laboratory include microscopic technique, isolation, cultivation and experimental applications of microbiology.

Course Outcomes
Upon completion of the course the student will be able to:

microscope: Demonstrate the proper care and usage of the microscope including oil immersion;
laboratory study of algae: Demonstrate methods of cultivation for algae;
protozoa: Recognize free living protozoa and parasitic protozoa from prepared slides and living materials;
microscopic study of microbes: Identify specimens from nature (water, soil, etc.), including yeast and molds;
cultivation of bacteria and other microorganisms: Perform pure culture isolation using streak plate and pour plate techniques and analyze broth and agar cultures;
bacteriological staining methods: Perform a simple stain, gram stain, and spore stain;
enzymatic reactions of microorganisms: Demonstrate the enzymatic reactions associated with carbohydrates, proteins lipids, and amino acids. Perform procedures isolating respiratory enzymes; and
isolation and identification of bacterial cultures: Perform techniques using differential plating and selective plating.

Concurrent Registration: BI 212
F (C, N, S)